Methods and approaches

Participants’ exposure to airborne pollutants, which include PM2.5 (particulate matter <2.5 micrometres), ozone (O₃), and nitrogen dioxide (NO₂), is assessed by an integrated approach combining Land Use Regression (LUR), Chemical...

The study involves urban analysis, both historical and present, in order to provide a deeper understanding of the impact of the urban environment on individuals who are daily exposed. Since...

Time spent in urban green areas (greenness exposure) is assessed using different procedures. Green and grey spaces surrounding the residential address of each participant are calculated based on the average...

For monitoring health and fitness parameters during the two weeks preceding sample collection, participants will wear a commercially available high-end wearable device, which continuously records oxygen saturation, skin temperature, breathing...

The study will enrol 6200 adult participants (aged >18 years) from the city of Legnano, a city in the Lombardy Region of Italy, with approximately 60,000 residents. Legnano offers a...

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) will be employed for the targeted biomonitoring of chemical pollutants in urine and plasma samples. The pollutants measured include:

Accurate characterization of RE 5-methylcytosine (5mC) methylation patterns using traditional next-generation sequencing (NGS), such as whole-genome bisulphite sequencing (WGBS), is challenging due to limitations in bioinformatic tools and the inherent...

Targeted methylation analysis will be applied during the tuning and validation phases of the MAMELI project to focus on specific REs identified in the discovery phase. A custom-designed targeted panel...